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Image Search Results
Journal: PLoS ONE
Article Title: Striatal Molecular Signature of Subchronic Subthalamic Nucleus High Frequency Stimulation in Parkinsonian Rat
doi: 10.1371/journal.pone.0060447
Figure Lengend Snippet: (A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. Microarray experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.
Article Snippet: For microarray experiments, amplification and fluorescent labeling of RNA was performed with the kit LIRLAK one color (Low Input RNA Linear Amplification Kit, Agilent) with starting amounts of 200 to 400 ng of total RNA. cRNAs were hybridized on Rat AGILENT 4×44 K microarrays and, after adequate washing, scanned with
Techniques: Microarray, Expressing